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Characterization of a mouse DNA clone containing an immunoglobulin variable region gene
Nucleic Acids Research
|June 1, 1978
Summary
Researchers cloned a mouse DNA fragment containing a Vlambda gene sequence. This immunoglobulin gene segment is distinct from the C-lambda gene, confirmed through DNA sequencing and restriction mapping.
Area of Science:
- Molecular Biology
- Immunogenetics
- Genomics
Background:
- Immunoglobulin light chain genes are crucial for adaptive immunity.
- Understanding gene organization and expression is key to immune system research.
- Previous studies suggested distinct V and C gene segments.
Purpose of the Study:
- To characterize a cloned mouse DNA fragment from embryonic tissue.
- To identify and confirm the presence of immunoglobulin Vlambda gene sequences.
- To investigate the relationship between Vlambda and Clambda gene sequences.
Main Methods:
- Gene cloning: Insertion of mouse DNA into a phage lambda vector.
- DNA characterization: Electron microscopy, restriction enzyme mapping, and partial DNA sequencing.
- Sequence homology analysis: Comparison with immunoglobulin light lambda chain mRNA.
Main Results:
- A 4.8 kilobase mouse DNA fragment was successfully cloned and characterized.
- A 400 base sequence within the fragment showed homology to immunoglobulin light lambda chain mRNA.
- Restriction mapping and sequencing confirmed the fragment contains a Vlambda gene sequence separate from any Clambda sequence.
Conclusions:
- The cloned DNA fragment contains a variable (Vlambda) gene sequence.
- This Vlambda gene sequence is physically separated from the constant (Clambda) gene sequence.
- Provides evidence for the distinct organization of immunoglobulin light chain genes.