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Related Experiment Videos

Multiple restricted origin of oligodendrocytes

N Spassky1, C Goujet-Zalc, E Parmantier

  • 1Biologie des Interactions Neurones/Glie, Institut National de la Santé et de la Recherche Médicale U-495, Université Pierre Marie Curie, Hôpital de la Salpêtrière, 75651 Paris Cedex 13, France.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
|October 8, 1998
PubMed
Summary
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The proteolipid protein (plp) gene and its product DM-20 are key to CNS myelin. Early plp/dm-20 expression identifies a distinct neural precursor lineage that generates oligodendrocytes, suggesting a plural origin for these cells.

Area of Science:

  • Neuroscience
  • Developmental Biology
  • Cell Biology

Background:

  • The proteolipid protein (plp) gene and its alternatively spliced product DM-20 are major components of central nervous system (CNS) myelin.
  • plp/dm-20 transcripts are expressed early in mouse development (embryonic day 9.5) by specific neuroepithelial cells.

Purpose of the Study:

  • To identify the neural precursors responsible for early plp/dm-20 expression.
  • To investigate the lineage and developmental potential of these early plp/dm-20 expressing cells.

Main Methods:

  • Generation of transgenic mice using a zeomycin resistance gene fused to a lacZ reporter under the control of plp regulatory sequences.
  • Analysis of beta-galactosidase expression patterns in vivo and in vitro.
  • Cell sorting and enrichment studies using zeomycin selection.

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Main Results:

  • Beta-galactosidase expression mirrored endogenous plp/dm-20 expression, identifying O4(+) pre-oligodendrocytes and RIP+ oligodendrocytes.
  • Zeomycin selection dramatically enriched O4(+) pre-oligodendrocytes, confirming oligodendroglial specification.
  • Early plp/dm-20 precursors differed from PDGFRalpha oligodendrocyte precursors in distribution and PDGF responsiveness.

Conclusions:

  • Early plp/dm-20 expression defines a specific neural precursor population that continuously expresses these myelin proteins.
  • These precursors are committed to the oligodendroglial lineage.
  • The findings suggest that oligodendrocytes originate from multiple distinct neural lineages, with early plp/dm-20 marking one such lineage.