Related Experiment Video
Updated: Oct 28, 2026

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
Published on: January 16, 2017
Four distinct regions in the auxiliary domain of heterogeneous nuclear ribonucleoprotein C-related proteins
W Jiang1, X Guo, V P Bhavanandan
1Department of Biochemistry and Molecular Biology H171, Pennsylvania State University College of Medicine, Hershey 17033, USA. wjiang@psu.edu
Abstract:
A 306 amino acid sequence deduced from a rabbit bladder cDNA is 98% identical to the human heterogeneous nuclear ribonucleoprotein (hnRNP) C2. The sequence comparison of the hnRNP C-related proteins reveals four distinct regions in the C-terminal auxiliary domain. The region next to the N-terminal RNA-binding domain is variable in length. Following the variable region, a basic region and a leucine zipper are conserved in all hnRNP C-related proteins including mouse and human Raly. Several Lys-Ser-Gly repeats are present in the basic region of the hnRNP C proteins. The C-terminal region is more divergent between hnRNP C and Raly. Signature sequences and possible functions are proposed for the different regions of the hnRNP C proteins.
Related Concept Videos
Conservation of Protein Domains Over Different Proteins
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to form...
The Nucleolus
Additional Subnuclear Structures
The nucleus contains many membrane-less subnuclear organelles or nuclear bodies, such as nucleoli, Cajal bodies, speckles, paraspeckles, etc. These nuclear...
Ribosomal RNA Synthesis
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
Rab Cascades
RNA Polymerase II Accessory Proteins

