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The effect of prolonged dextran administration in guinea pigs
Physiologie (Bucarest)
|April 1, 1978
Summary
Daily intraperitoneal injections of Dextran 70 in guinea pigs lowered plasma proteins and antibody concentrations. Red blood cell counts and hemoglobin also decreased, suggesting potential impacts on hematopoiesis and immune function.
Area of Science:
- Biochemistry
- Immunology
- Hematology
Background:
- Dextran is a polysaccharide used in medical applications.
- Its effects on plasma proteins, immune response, and hematopoiesis require detailed investigation.
Purpose of the Study:
- To evaluate the impact of prolonged intraperitoneal Dextran 70 administration on plasma protein levels, colloid osmotic pressure, and immune parameters in guinea pigs.
Main Methods:
- Guinea pigs received daily intraperitoneal injections of 6% Dextran 70 in 0.9% NaCl for 27 days.
- Plasma protein levels, colloid osmotic pressure, antibody concentrations, and red blood cell parameters were analyzed.
- Immunodiffusion and bone marrow analysis were performed.
Main Results:
- Dextran 70 administration led to a significant decrease in plasma protein levels.
- Colloid osmotic pressure remained comparable to controls.
- Concentrations of typhus O antibodies and precipitation lines were reduced.
- Red blood cell count and hemoglobin levels decreased, correlating with a reduced erythroblastic series in bone marrow.
Conclusions:
- Prolonged intraperitoneal Dextran 70 administration affects plasma protein homeostasis and immune function.
- Hematological changes suggest an impact on red blood cell production.
- Minimal histological changes in liver and kidney indicate these organs' role in Dextran elimination.