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Potato virus A detection by reverse transcription-polymerase chain reaction
N Cerovská1, K Petrzik, T Moravec
1Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
Acta Virologica
|October 14, 1998
Abstract:
Simple and reliable procedure for sample preparation and reverse transcription-polymerase chain reaction (RT-PCR) detection of potato virus A (PVA) is described. PVA-specific primers used in the RT-PCR defined a target sequence of 321 bp and did not produce amplification product(s) with potato virus Y.