Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural changes in hemoglobin during adsorption to solid surfaces: effects of pH, ionic strength, and ligand

F Höök1, M Rodahl, B Kasemo

  • 1Department of Biochemistry and Biophysics, Göteborg University and Chalmers University of Technology, Medicinaregatan 9C, SE-413 90 Göteborg, Sweden. fredrik.hook@bcbp.gu.se

Proceedings of the National Academy of Sciences of the United States of America
|October 15, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Clinical and epidemiological features of psoriasis exacerbations in children with SARS-CoV-2 infection.

Journal of the European Academy of Dermatology and Venereology : JEADV·2023
Same author

SYMPTOMATOLOGY AND TREATMENT OF COVID-19 AFFECTING SKIN APPENDAGES: A NARRATIVE REVIEW BEYOND COVID-TOES.

Georgian medical news·2022
Same author

Children with psoriasis and COVID-19: factors associated with an unfavourable COVID-19 course, and the impact of infection on disease progression (Chi-PsoCov registry).

Journal of the European Academy of Dermatology and Venereology : JEADV·2022
Same author

Partial Ribosomal Nontranscribed Spacer Sequences Distinguish Rhagoletis zephyria (Diptera: Tephritidae) From the Apple Maggot, R. pomonella.

Journal of economic entomology·2022
Same author

Monitoring of single and double lipid membrane formation with high spatiotemporal resolution using evanescent light scattering microscopy.

Nanoscale·2016
Same author

Ferritin-supported lipid bilayers for triggering the endothelial cell response.

Colloids and surfaces. B, Biointerfaces·2016

Quartz Crystal Microbalance (QCM) analysis reveals two distinct states of adsorbed hemoglobin proteins on gold surfaces. This technique differentiates between rigidly bound, denatured proteins and loosely bound, native-like proteins, offering insights into protein adsorption mechanisms.

Area of Science:

  • Biophysics
  • Surface Science
  • Materials Chemistry

Background:

  • Protein adsorption on surfaces is crucial for biosensor development and understanding biological interactions.
  • Hemoglobin (Hb) adsorption behavior is complex and influenced by protein structure and surface properties.
  • Distinguishing between native and denatured protein states upon adsorption is challenging.

Purpose of the Study:

  • To investigate the viscoelastic properties of adsorbed hemoglobin (met-hemoglobin and carboxyhemoglobin) on a hydrophobic surface.
  • To differentiate between various states of adsorbed proteins using simultaneous mass and viscoelastic measurements.
  • To elucidate the mechanisms of protein adsorption and layer formation on self-assembled monolayers.

Main Methods:

  • Utilized Quartz Crystal Microbalance (QCM) with simultaneous frequency (mass) and dissipation (viscoelasticity) measurements.

Related Experiment Videos

  • Studied the adsorption of met-hemoglobin (met-Hb) and carboxyhemoglobin (HbCO) on a methyl-terminated thiol monolayer on gold.
  • Analyzed DeltaD vs. Deltaf graphs to identify different adsorbed protein states below the isoelectric points.

    • Main Results:

    • Observed two distinct phases in the DeltaD vs. Deltaf graphs, indicating two states of adsorbed proteins with different viscoelastic properties.
    • The first phase correlated with rigidly bound, likely denatured proteins, while the second phase indicated loosely bound proteins.
    • The second layer of proteins desorbed upon chemical modification (met-Hb to HbCO), suggesting a native-like state.

    Conclusions:

    • Simultaneous QCM measurements provide unique insights into the viscoelastic changes and conformational states of adsorbed proteins.
    • The study demonstrates that adsorbed proteins can exist in different states, including native-like configurations.
    • Understanding these adsorption mechanisms is vital for designing stable and functional protein-surface interfaces.