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In situ localisation of Yersinia enterocolitica by catalysed reported deposition signal amplification
P T Odinot1, J F Meis, J A Hoogkamp-Korstanje
1Department of Medical Microbiology, University Hospital Nijmegen, The Netherlands.
Aim:
The sensitive detection of pathogenic Yersinia enterocolitica in paraffin embedded tissue sections by in situ hybridisation (ISH).
Methods:
Y enterocolitica infected cell lines, rat spleens, and patient biopsy specimens were used to compare conventional ISH, immune fluorescence assay (IFA) detection, and catalysed reporter deposition (CARD) signal amplification ISH.
Results:
CARD-ISH was shown to be more sensitive then conventional ISH and had a comparable sensitivity to IFA. In contrast to IFA, CARD-ISH preserved good tissue morphology.
Conclusions:
CARD-ISH appeared to be a fast and sensitive ISH method for detecting Y enterocolitica in routinely processed tissue sections. Application of this method allows the combination of routine detection and cellular localisation of the pathogen within the infected tissue.