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Circulating L-selectin levels and endothelial CD34 expression in inflammatory bowel disease
J B Seidelin1, B Vainer, T Horn
1Department of Internal Medicine CF, Glostrup Hospital, University of Copenhagen, Denmark.
The American Journal of Gastroenterology
|October 15, 1998
Summary
Soluble L-selectin (sL-selectin) is linked to ulcerative colitis (UC) activity but not Crohn's disease (CD). CD34 expression did not explain this difference, suggesting neutrophil activation or chemokine secretion influences sL-selectin levels in inflammatory bowel disease.
Area of Science:
- Gastroenterology
- Immunology
- Molecular Biology
Background:
- Soluble L-selectin (sL-selectin) concentrations correlate with disease activity in ulcerative colitis (UC) but not Crohn's disease (CD).
- This discrepancy suggests disease-specific regulation of L-selectin ligands may be involved.
Purpose of the Study:
- To compare circulating sL-selectin levels.
- To evaluate L-selectin ligand CD34 expression in the colon.
- To assess inflammatory bowel disease (IBD) activity.
Main Methods:
- Enzyme-linked immunosorbent assay (ELISA) for serum sL-selectin.
- Immunohistochemical analysis of CD34 expression in colon biopsies.
- Clinical semiquantitative scale for disease activity assessment.
Main Results:
- sL-selectin levels significantly increased with UC disease activity (p < 0.001), but not in CD (p > 0.05).
- UC patients showed significantly lower sL-selectin in quiescent disease and higher levels in severe disease compared to controls.
- CD34 expression was elevated in both UC and CD patients compared to controls (p < 0.05).
Conclusions:
- Disease-specific CD34 regulation does not explain the differential sL-selectin behavior in UC and CD.
- Low sL-selectin in quiescent disease may be due to neutrophil activation.
- Increased sL-selectin in severe disease activity might be linked to chemokine secretion.