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[Oxygen free radical on interleukin-1 activity of hemorrhage/resuscitation rat]
Objective:
To investigate the effects of oxygen free radical on the enhancement of IL-1 activity in vivo and in vitro on hemorrhagic and resuscitated rat.
Methods:
30% of rats total blood volume was withdraw by carotid artery catheter and resuscitated with 2 times of lost blood volume 1 h later.
Results:
Plasm IL-1 activity and MDA content increased and SOD activity decreased significantly 1-4 hours after resuscitation. There was a marked correlation between IL-1 activity and MDA content as well as SOD activity. Treatment with SOD as resuscitation prevented the postresuscitation increase in plasma IL-1 activity significantly. Hemorrhage and resuscitation also caused significant decrease of SOD activity and elevation of MDA in peritoneal macrophage 2 hours after resuscitation. After preincubation with SOD for 1 hour, the macrophage presented a much lowered capacity to release IL-1.
Conclusion:
Oxygen free radical may be one of the most important factors that contribute to elevation of IL-1 level after hemorrhage and resucitation.