Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Photosensitive pigments formed with rat opsin

R Crouch

    Investigative Ophthalmology
    |October 1, 1976
    PubMed
    Summary

    Researchers created photosensitive pigments from rat opsin and various retinal isomers. These pigments showed similar spectral properties and photosensitivity to bovine counterparts, despite slower formation rates.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Black Hole Spectroscopy and Tests of General Relativity with GW250114.

    Physical review letters·2026
    Same author

    GW250114: Testing Hawking's Area Law and the Kerr Nature of Black Holes.

    Physical review letters·2025
    Same author

    Improving cosmological reach of a gravitational wave observatory using Deep Loop Shaping.

    Science (New York, N.Y.)·2025
    Same author

    Squeezing the quantum noise of a gravitational-wave detector below the standard quantum limit.

    Science (New York, N.Y.)·2024
    Same author

    Cardiac SABR: Image matching techniques for accurate treatment delivery.

    Radiography (London, England : 1995)·2024
    Same author

    'Inappropriate attended' in A&E.

    Nursing standard (Royal College of Nursing (Great Britain) : 1987)·2016

    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Vision Science

    Background:

    • Photosensitive pigments, like rhodopsin, are crucial for vision.
    • Understanding pigment formation and properties aids in studying visual processes.
    • Rat opsin serves as a model system for investigating pigment assembly.

    Purpose of the Study:

    • To investigate the in vitro formation of photosensitive pigments using rat opsin.
    • To characterize the spectral properties and photosensitivity of newly formed pigments.
    • To compare these properties with known bovine visual pigments.

    Main Methods:

    • Rat retinal rods were used, either in suspension or as digitonin extracts.
    • Opsin was combined with different retinal isomers (11-cis, 9-cis, 9,13-dicis) to form pigments.
    • Absorption spectra and photosensitivity assays were performed on the generated pigments.

    Main Results:

    • Rhodopsin, isorhodopsin I, and isorhodopsin II were successfully synthesized in vitro.
    • An analogue of isorhodopsin I was formed using a modified retinal molecule.
    • The generated rat pigments exhibited spectral characteristics and photosensitivity comparable to bovine pigments.
    • Formation rates for rat pigments were observed to be significantly slower than bovine pigments.

    Conclusions:

    • Rat opsin can form functional photosensitive pigments in vitro.
    • The spectral and photosensitive properties of these pigments are conserved across species.
    • Differences in formation rates suggest variations in molecular interactions or stability.

    Related Experiment Videos