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[Separation and preparation of indole alkaloids in Lycorma delicatula White. by HPLC]
1Institute of Chinese Materia Medica, China Academy of Traditional Chinese Medicine, Beijing.
Abstract:
A HPLC method for separating and preparing indole alkaloids is described. HPLC conditions for analysis: BIO-RAD series 700 HPLC, model 700 data station, UV: model 1749 UV-VIS monitor, column: BIO-RAD Hi-pore RP318, 250 mm x 10 mm, mobile phase: 80% methanol-H2O(gradient), flow rate: 1.5 ml/min, detection wavelength: 254 nm. On the basis of spectral (1HNMR, 13CNMR, H-H COSY, MS, DEPT) and chemical evidence, the structures of two compounds were elucidated as beta-yohimbine (yohimban-16-carboxylic acid-17-hydroxy methylester (3 alpha, 16 alpha, 17 beta)) and ajmalicine (oxayohimban-16-carboxylic acid-16,17-didehydro-19-ethyl methyl ester (19 alpha)).