Effect of treatment with buffalo follicular fluid on ovarian function in goats

Oestrus was synchronised in 15 nondescript goats with two injections of 7.5 mg luprostiol given 11 days apart. They were randomised into two groups; nine (group 1) received 3 ml charcoal-extracted buffalo follicular fluid at 12 hour intervals on days 12 to 15 of the cycle and six (group 2) received an equal volume of normal saline at the same times. Luteolysis was induced 96 hours after the treatments began by a single injection of 7.5 mg luprostiol. The onset of oestrus was detected using a vasectomised buck and the ovarian response was determined by visual observation of the ovaries following a midventral laparotomy performed five or six days after oestrus. In the goats of group 1, oestrus occurred 99.3 (4.13) hours after the injection of luprostiol, and in the goats of group 2 after 68.0 (6.7) hours. Group 1 does had significantly more ovulations (2.56 [0.29]) and large (> or = 5 mm diameter) unovulated follicles (2.77 [0.40]) than the does of group 2 which had 1.83 (0.16) ovulations and 0.50 (0.34) large unovulated follicles.