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The H19 transcript is associated with polysomes and may regulate IGF2 expression in trans

Y M Li¹, G Franklin, H M Cui

¹Department of Animal Development & Genetics, Uppsala University, Norbyvägen 18A, S-752 36 Uppsala, Sweden.

The Journal of Biological Chemistry

|October 17, 1998

Summary

The H19 gene transcript, previously thought to be non-functional, is found on polysomes. This suggests H19 may regulate IGF2 expression, challenging existing scientific dogma.

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Area of Science:

Genetics
Molecular Biology
Gene Regulation

Background:

The H19 gene produces a transcript lacking a conserved open reading frame.
Transcriptional elements of H19 influence the neighboring Igf2 gene in cis.
The cytoplasmic H19 transcript was widely considered functionally inert.

Purpose of the Study:

To investigate the functional role of the H19 transcript.
To explore potential trans-regulatory functions of H19.
To challenge the prevailing view of H19's non-functionality.

Main Methods:

Analysis of H19 transcript association with polysomes across various cell types in mice and humans.
Examination of reciprocal correlations between H19 and IGF2 mRNA levels.
Assessment of IGF2 mRNA translatability.

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Main Results:

The H19 transcript is associated with polysomes in multiple cell types from both mouse and human.
A reciprocal correlation was observed between cytoplasmic H19 and IGF2 mRNA levels.
This correlation extended to IGF2 mRNA translatability, suggesting a trans-regulatory role.

Conclusions:

The H19 transcript is not functionally inert and associates with the translational machinery.
H19 may act as a trans-acting antagonist of IGF2 expression.
These findings challenge the established dogma regarding H19 function and have implications for Beckwith-Wiedemann syndrome.