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A 7.4-A projection structure of outer membrane phospholipase A from Escherichia coli by electron crystallography
E J Boekema1, M Stuart, R I Koning
1Groningen Biomolecular Sciences and Biotechnology Institute, University of Groningen, Nijenborgh 4, Groningen, NL-9747 AG, The Netherlands.
Journal of Structural Biology
|October 17, 1998
Abstract:
Outer membrane phospholipase A (OMPLA) is one of the few enzymes present in the outer membrane of Escherichia coli. Two-dimensional crystals of OMPLA were grown by reconstitution of purified protein into lipid bilayers via detergent dialysis and were studied by electron crystallography. A 7.4-A projection map reveals OMPLA molecules exhibiting an oval-shaped domain of 30 x 20 A resembling the beta-barrel structure characteristic of porins, which is associated with a 25-A elongated domain of lower density.