Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Immunoelectron microscopic study for polyamines

K Fujiwara1, G Bai, T Kitagawa

  • 1Faculty of Pharmaceutical Sciences, Nagasaki University, Nagasaki, Japan.

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
|October 17, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Twenty years of experience with lesions involving the temporomandibular joint and adjacent structures complex.

International journal of oral and maxillofacial surgery·2025
Same author

Diminished GALNS activity in induced pluripotent stem cells of mucopolysaccharidosis IVA caused by compound p.S162Y and p.C165F mutation.

QJM : monthly journal of the Association of Physicians·2024
Same author

Exogenous citric acid enhances drought tolerance in tobacco (Nicotiana tabacum).

Plant biology (Stuttgart, Germany)·2021
Same author

The role of protein restriction and interaction with antibiotics in the regulation of compensatory growth in pigs: growth performance, serum hormone concentrations, and messenger RNA levels in component tissues of the endocrine growth axis.

Domestic animal endocrinology·2020
Same author

MiR-195 alleviates ulcerative colitis in rats via MAPK signaling pathway.

European review for medical and pharmacological sciences·2020
Same author

A Single Amino Acid Substitution in the Intervening Region of 129K Protein of Cucumber Green Mottle Mosaic Virus Resulted in Attenuated Symptoms.

Phytopathology·2019
Same journal

Expression and Spatial Localization of Glycosaminoglycan Types in Cartilage From Different Anatomical Locations.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Aquaporin-4 Localization in Terminal Glial Cells of Human Mechanosensory Corpuscles: An Immunohistochemical Study of Penile Tissues.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Seeing, Interpreting, and Trusting Images in the Age of Artificial Intelligence.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

P63 and Tight Junctions Targeted for Therapy in Human Salivary Duct Adenocarcinoma Cells.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

Human iPSC-Derived Blood Vessel Organoids for Studying Chronic Hypoxia-Induced Microvascular Dysfunction.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
Same journal

From Manual to Macro: A Reproducible Fiji Workflow for Semi-automated Collagen Fibril Diameter Quantification in Transmission Electron Microscopy.

The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society·2026
See all related articles

Polyamines (PAs) are crucial cell metabolites. This study found PAs primarily on ribosomes in rat neurons, suggesting their direct role in protein biosynthesis and translation.

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Neuroscience

Background:

  • Polyamines (PAs) are vital polycationic metabolites in cells.
  • Their precise function in DNA, RNA, and protein biosynthesis is unclear due to unknown subcellular localization.
  • Understanding PA localization is key to elucidating their biological roles.

Purpose of the Study:

  • To determine the subcellular localization of polyamines (PAs) in neurons.
  • To investigate the relationship between PA localization and protein biosynthesis.

Main Methods:

  • Immunoelectron microscopy was employed to visualize PA distribution.
  • The study focused on neurons within the rat medulla oblongata's lateral reticular nucleus.

Main Results:

Related Experiment Videos

  • Polyamines (PAs) were predominantly found on free and attached ribosomes of the rough endoplasmic reticulum.
  • Nuclei, axons, and nerve endings showed no detectable PA.
  • This specific localization suggests a direct role in cellular machinery.

Conclusions:

  • Polyamines (PAs) are localized on ribosomes, indicating a role in protein biosynthesis.
  • The findings suggest PAs are integral components of biologically active ribosomes.
  • PAs are likely closely involved in the translation process of protein synthesis.