The inactivation kinetics of papain by guanidine hydrochloride: a re-analysis

The kinetic theory of the substrate reaction during modification of enzyme activity has been applied to study the inactivation kinetics of enzymes by denaturant. However, an important problem related to the determination of the inactivation rate constants has not been considered in a previous publication (Xiao, et al., Biochim. Biophys. Acta, 1164 (1993) 54-60). In most denaturation experiments, the high concentrations of denaturants may greatly affect the kinetic behavior of the system to preclude the use of the kinetic parameters determined in the absence of denaturant. In the present study, the kinetic equation of substrate reaction in presence of denaturant has been derived. A re-examination of the effect of high concentrations of guanidine hydrochloride on the inactivation of papain, taking into consideration the effect of high concentrations of guanidine hydrochloride on the Michaelis constant, showed that, for papain, the substrate gives no protection on its inactivation. It is the purpose of the present communication to stress the importance of observing the effect of the denaturant on the kinetic parameters for kinetic analysis of enzyme inactivation by denaturants generally.