Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

A rapid (20 h) solid screening medium for detecting methicillin-resistant Staphylococcus aureus

P L Perry1, G W Coombs, J D Boehm

  • 1Department of Microbiology and Infectious Diseases, Royal Perth Hospital, Western Australia.

The Journal of Hospital Infection
|October 20, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Antimicrobial-resistant Staphylococcus aureus isolated from Australian wildlife admitted to a veterinary hospital.

Australian veterinary journal·2026
Same author

Global Epidemiology and Evolutionary History of Staphylococcus aureus ST45.

Journal of clinical microbiology·2020
Same author

Evaluation of the Haemophilus influenzae EUCAST and CLSI disc diffusion methods to recognize aminopenicillin and amoxicillin/clavulanate resistance.

The Journal of antimicrobial chemotherapy·2020
Same author

Marked increase in community-associated methicillin-resistant <i>Staphylococcus aureus</i> infections, Western Australia, 2004-2018.

Epidemiology and infection·2020
Same author

Transmission of highly virulent community-associated MRSA ST93 and livestock-associated MRSA ST398 between humans and pigs in Australia.

Scientific reports·2017
Same author

Characterization of a novel staphylococcal cassette chromosome composite island from community-associated MRSA isolated in aged care facilities in Western Australia.

The Journal of antimicrobial chemotherapy·2016
Same journal

The relationship between asymptomatic bacteriuria and surgical site infection in hip fracture patients: a systematic review and meta-analysis.

The Journal of hospital infection·2026
Same journal

Status of Hand Hygiene Implementation Using Multimodal Strategy in Public Hospitals of Ethiopia: A Multi-Center Study.

The Journal of hospital infection·2026
Same journal

Diagnostic Stewardship for optimizing blood culture practices: A Systematic Review of clinical practice guidelines and evidence-based recommendations.

The Journal of hospital infection·2026
Same journal

Identification of risk factors for hospital-onset bacteremia to inform a routine data based risk prediction - an umbrella review.

The Journal of hospital infection·2026
Same journal

Healthcare-associated Infections in the Middle East: A Systematic Review and Meta-Analysis of Point-Prevalence Studies.

The Journal of hospital infection·2026
Same journal

Expression of concern: Postoperative serum amyloid A as a primary marker in a predictive model for ventilator-associated pneumonia in elderly patients with acute ischaemic stroke undergoing endovascular therapy with general anaesthesia [Journal of Hospital Infection, pre-proof, available online 8 July 2025].

The Journal of hospital infection·2026
See all related articles

This study introduces a rapid screening medium for methicillin-resistant Staphylococcus aureus (MRSA). The new method allows for reliable MRSA detection or exclusion within 20 hours, significantly speeding up diagnosis.

Area of Science:

  • Microbiology
  • Clinical Diagnostics
  • Antimicrobial Resistance

Background:

  • Accurate and rapid detection of methicillin-resistant Staphylococcus aureus (MRSA) is crucial for effective patient management and infection control.
  • Existing diagnostic methods can be time-consuming, delaying appropriate treatment and isolation protocols.

Purpose of the Study:

  • To evaluate the sensitivity and reliability of a novel solid screening medium for MRSA detection.
  • To determine the optimal incubation period for accurate MRSA identification using this medium.

Main Methods:

  • Utilized a methicillin aztreonam mannitol salt agar screening medium.
  • Incubated the medium for 20 hours to observe colony growth.
  • Performed coagulase testing on positive isolates for provisional MRSA identification.

Related Experiment Videos

Main Results:

  • The screening medium demonstrated high sensitivity and reliability in detecting MRSA.
  • Visible MRSA colonies or complete exclusion of MRSA was achieved within a 20-hour incubation period.
  • Provisional 'possible MRSA' reports were possible within 26-30 hours post-swab collection, including low-expression MRSA strains.
  • The medium effectively suppressed susceptible staphylococci and beta-lactamase hyperproducers.

Conclusions:

  • Methicillin aztreonam mannitol salt agar is an effective and rapid screening tool for MRSA.
  • This medium significantly reduces the time required for MRSA diagnosis compared to conventional methods.
  • The 20-hour incubation period allows for timely clinical decisions regarding MRSA infections.