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Periotron 8000: calibration characteristics and reliability

M Ciantar1, D J Caruana

  • 1Department of Dental Surgery, University of Malta, Msida, Malta.

Journal of Periodontal Research
|October 20, 1998
PubMed
Summary

The Periotron 8000 accurately quantifies fluid volumes, but calibration depends on fluid composition and standardized filter paper strip placement. Its calibration remains consistent over a week.

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Area of Science:

  • Biomedical instrumentation
  • Fluid dynamics
  • Analytical chemistry

Background:

  • The Periotron instrument measures submicrolitre fluid volumes on filter paper strips.
  • Previous models include the Periotron 600 (1976), 6000 (1983), and 8000 (1995).

Purpose of the Study:

  • To evaluate the calibration characteristics and reliability of the Periotron 8000 for the first time.
  • To analyze quantitative and qualitative fluid composition effects on Periotron readings.

Main Methods:

  • Tested Periotron 8000 with de-ionised water, human serum, fetal bovine serum, and its ultrafiltrate.
  • Recorded Periotron readings for fluid volumes from 0-1.0 microliters.
  • Plotted average readings against fluid volumes and analyzed qualitative changes.

Main Results:

  • Calibration fluid composition, particularly protein content, influences Periotron scores.
  • Standardized positioning of the filter paper strip is crucial for consistent results.
  • Periotron 8000 calibration demonstrated consistency over a one-week period.

Conclusions:

  • The Periotron 8000 is a reliable instrument for submicrolitre fluid quantification.
  • Standardization protocols are necessary for accurate and reproducible measurements.
  • Further research may explore a wider range of fluid compositions and longer-term calibration stability.

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