Liquid chromatographic determination of biotin in multivitamin-multimineral tablets

A reproducible reverse phase high pressure liquid chromatography (RP-HPLC) method for the determination of biotin in multivitamin-multimineral tablets has been developed and validated. This method involves reverse phase separation of the component monitored by absorbance at 200 nm wavelength. The method has excellent precision and accuracy with S.D. 0.83 and 2.9%, respectively. The established linearity range was 0.5-2 micrograms ml-1 (r2 > 0.9999). The recovery of biotin from spiked placebo was > 97% over the linear range. The extraction procedure is simple and the HPLC conditions separate biotin from its degradation products and excipients. The method has been successfully used in determining biotin content in 4 brands of commercially available multivitamin-multimineral tablets.