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Related Experiment Videos

Cohesion between sister chromatids must be established during DNA replication

F Uhlmann1, K Nasmyth

  • 1Research Institute of Molecular Pathology, Dr. Bohr-Gasse 7, A-1030, Vienna, Austria.

Current Biology : CB
|October 21, 1998
PubMed
Summary

Sister chromatid cohesion, crucial for accurate cell division, must be established during DNA replication. The protein Scc1p (sister chromatid cohesion 1p) is vital for establishing and maintaining this cohesion.

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Area of Science:

  • Cell Biology
  • Molecular Biology
  • Genetics

Background:

  • Sister chromatid cohesion is essential for proper chromosome segregation during cell division.
  • Cohesins, including Smc1p, Smc3p, and Scc1p, regulate this cohesion in Saccharomyces cerevisiae.
  • Defects in cohesin proteins lead to premature sister chromatid separation and chromosome missegregation.

Purpose of the Study:

  • To investigate the precise timing of Scc1p (sister chromatid cohesion 1p) function in establishing sister chromatid cohesion.
  • To determine if Scc1p is required during S phase for the establishment of cohesion.

Main Methods:

  • Utilized a galactose-inducible promoter system to control Scc1p expression timing.
  • Compared sister chromatid cohesion in cells with normal Scc1p expression versus delayed Scc1p expression.

Related Experiment Videos

  • Observed Scc1p recruitment to chromosomes and its effect on cohesion.

    • Main Results:

    • Scc1p is synthesized and recruited to chromosomes at the start of S phase in normal cell cycles.
    • Delayed Scc1p synthesis after S phase completion allowed chromosome binding but not cohesion establishment.
    • This indicates that Scc1p's role in cohesion requires its presence during S phase.

    Conclusions:

    • Sister chromatid cohesion must be established during DNA replication, potentially after replication fork passage.
    • Scc1p and other cohesins are necessary for both establishing cohesion during S phase and maintaining it during mitosis.
    • The establishment of sister chromatid cohesion during S phase is a critical, previously overlooked process.