[Leukocyte reactions "in vitro" to human spermatozoa in infertile females]

Leucocyte migration test was done in 55 couples facing fertility problems for detection of antispermatozoal cellular hypersensitivity. In 29 cases out of 55, the sterility was unexplained and in 26 cases out of 55, various infertility factors were found. In this study, wife's leucocytes separated on Ficoll-Urografin gradient were incubated (4.10(6) per capillary) with washed fresh husband's spermatozoa suspended at 7.10(6)/ml in M 199 and 20 p. 100 fetal bovine serum. The results were read after 18h. Two kinds of migration inhibition were found: 1) Specific wives leucocyte migration inhibition in 4 out of 55 cases, all of them unexplained infertility cases. 2) Leucocyte migration inhibition not related to wife's immunity state and possibly due to a direct effect of a toxic or inhibitory property of some husband's spermatozoa in 22 out of 55 cases, 11/19 cases with known male factors, and 11/36 cases without any male factor. In the follow up, only 1 woman out of the 22 couples with husband's spermatozoal inhibitory factor was pregnant later, while in the group of 29 women in which normal leucocyte spreading was found, 8 women conceived later. No woman out of the 4 suspected of antispermatozoal delayed hypersensitivity have conceived. Attention must be paid to the possible role of delayed hypersensitivity to sperm in unexplained infertility and also to the occurrence of spermatozoal toxic factor and its possible implications on human infertility.