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Patch-clamp, ion-sensing, and glutamate-sensing techniques to study glutamate transport in isolated retinal glial
B Billups1, M Szatkowski, D Rossi
1Department of Physiology, University College London, England.
Methods in Enzymology
|October 21, 1998
Abstract:
We have described how a combination of electrical, ion-sensing, and glutamate-sensing techniques has advanced our understanding of glutamate uptake into isolated salamander retinal glial cells. The next steps in understanding glutamate transport will inevitably depend strongly on molecular biological methods, as described elsewhere in this book, but will also require more detailed study of transporters in their normal environment, perhaps by using patch-clamping or imaging techniques to study cells in situ.