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Solubilization of IgA immune precipitates by complement

G W Miller

    Journal of Immunology (Baltimore, Md. : 1950)
    |October 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    The complement system can dissolve immune complexes formed with IgA antibodies, similar to IgG and IgM. This demonstrates the complement-mediated solubilization effect is a general phenomenon across antibody classes.

    Area of Science:

    • Immunology
    • Complement System Biology

    Background:

    • The complement system is known to solubilize immune precipitates formed with IgG and IgM antibodies.
    • The mechanism and scope of this complement-mediated solubilization effect across different antibody isotypes require further investigation.

    Purpose of the Study:

    • To investigate whether the complement system can solubilize immune precipitates formed with IgA myeloma proteins.
    • To determine if this solubilization effect is dependent on specific complement pathways or ions.

    Main Methods:

    • Preparation of immune precipitates using mouse myeloma proteins (TEPC 15 and MOPC 315) with specific antigens (PC-KLH and DNP-BSA).
    • Incubation of these precipitates with fresh or inactivated mouse serum (zymosan- or heat-inactivated).
    • Assessment of solubilization in the presence or absence of Ca++ ions.

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    Main Results:

    • Complement system successfully solubilized immune precipitates formed with IgA myeloma proteins.
    • Solubilization occurred with fresh serum but not with inactivated serum, indicating a requirement for active complement components.
    • The rate of solubilization was not affected by the removal of Ca++ ions, suggesting the alternative complement pathway is involved.

    Conclusions:

    • Complement-mediated solubilization of immune precipitates is not limited to IgG and IgM but also extends to IgA antibodies.
    • The complement system's ability to solubilize immune complexes is a general phenomenon, independent of antibody class and antigen type.
    • The alternative complement pathway plays a significant role in this solubilization process.