Enrichment of PHA transformed lymphocytes in samples containing mixed populations

A dextran density gradient centrifugation method has been used to separate PHA transformed lymphocytes from nontransformed lymphocytes obtained from rat blood. Fifty-four percent of the transformed lymphocytes were recovered in the fraction at a density of 1.0620 g/ml. Eighty-five percent of the cells in this fraction were transformed cells while the remainder were non-transformed lymphocytes.