Synaptic density in the arcuate nucleus of female rats approaching middle age

Synaptic number and synapses/neuronal cell membrane were evaluated from ultrastructural micrographs of the arcuate nucleus from 90-, 120-, 180-, and 240-day female rats grouped into ovary-intact or ovariectomized animals treated with peanut oil vehicle or estradiol benzoate (10 microg/100 g body weight) for 3 days. In ovary-intact rats, synaptic density was significantly less in middle aged 240 day animals than in 90-, 120-, or 180-day animals with greatest decrease occurring between 180- and 240-day animals. Ovary-intact and ovariectomized animals treated with estradiol benzoate had significantly higher sera estradiol levels, but the estradiol was ineffective in increasing synaptic density in the middle aged animals. Logistical regression confirmed a correlation between a decrease in synapses and increasing age but not estradiol treatment.