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beta-Lactamase activity in Chromobacterium violaceum

W E Farrar, N M O'dell

    The Journal of Infectious Diseases
    |September 1, 1976
    PubMed
    Summary

    A Chromobacterium violaceum strain produced beta-lactamase, an enzyme that breaks down antibiotics. This enzyme

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    Area of Science:

    • Microbiology
    • Pharmacology
    • Biochemistry

    Background:

    • A fatal infection caused by Chromobacterium violaceum.
    • Detection of beta-lactamase production in this bacterial species.

    Purpose of the Study:

    • Characterize the beta-lactamase produced by Chromobacterium violaceum.
    • Determine the enzyme's activity against various beta-lactam antibiotics.
    • Investigate the genetic basis of beta-lactamase production.

    Main Methods:

    • Isolation and culture of Chromobacterium violaceum.
    • Assay of beta-lactamase activity under different conditions (drug-free vs. penicillin G).
    • Testing enzyme inhibition and substrate specificity.
    • Antibiotic susceptibility testing.
    • Genetic analysis of beta-lactamase gene location.

    Main Results:

    • Beta-lactamase production was inducible by penicillin G.
    • The enzyme showed primary activity against cephalosporins.
    • It was sensitive to cloxacillin but resistant to p-chloromercuribenzoate.
    • Chromobacterium violaceum exhibited variable susceptibility to beta-lactam antibiotics, with resistance to 11 tested agents.
    • Beta-lactamase production was linked to chromosomal genes.

    Conclusions:

    • The identified beta-lactamase shares characteristics with enzymes found in Pseudomonas aeruginosa.
    • Chromobacterium violaceum can develop antibiotic resistance through inducible beta-lactamase production.
    • Understanding this mechanism is crucial for treating infections caused by this pathogen.

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