Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Epithelial stratification in the developing chick cornea

The Journal of Experimental Zoology

|November 1, 1976

Summary

This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Age and cell type influences on nerve--non-nerve contact interactions.

Brain research·1986

Same author

Differential response to contact during embryonic nerve-nonnerve cell interactions.

Cell motility·1983

Same author

Responses to cell contacts between growth cones, neurites and ganglionic non-neuronal cells.

Journal of neurocytology·1980

Same author

Veils, mounds, and vesicle aggregates in neurons elongating in vitro.

Experimental cell research·1979

Same author

Axon initiation and growth cone regeneration in cultured motor neurons.

Experimental cell research·1978

Same author

Normal branching, induced branching, and steering of cultured parasympathetic motor neurons.

Experimental cell research·1978

Same journal

Myosin expression in the developing ascidian embryo.

The Journal of experimental zoology·2012

Same journal

Regulation in the chick allantois.

The Journal of experimental zoology·2010

Same journal

A serologic study of conjugation in Paramecium bursaria.

The Journal of experimental zoology·2010

Same journal

Studies on flagellates from domesticated birds; the behavior of Trichomonas gallinarum in culture.

The Journal of experimental zoology·2010

Same journal

Experiments on the formed inclusions of the amphibian egg; the effect of pH and electrolytes on yolk and lipochondria.

The Journal of experimental zoology·2010

Same journal

Production of strophosomy in the chick embryo by local applications of colchicine.

The Journal of experimental zoology·2010

See all related articles

Embryonic corneal epithelial stratification involves dynamic changes in cell division orientation. Mitotic spindles shift from horizontal to vertical alignment during layer formation, aiding stratified tissue development.

Area of Science:

Developmental Biology
Cell Biology
Epithelial Biology

Background:

Epithelial stratification is crucial for tissue development and function.
Understanding the cellular mechanisms driving stratification is essential.
The embryonic chick corneal epithelium serves as a model for studying stratification.

Purpose of the Study:

To investigate the process of epithelial stratification in the embryonic chick corneal epithelium.
To analyze DNA synthetic activity, cell density, and mitotic spindle orientation during stratification.
To correlate cellular behaviors with the formation of stratified layers.

Main Methods:

Observation of DNA synthetic activity in individual cell layers.
Measurement of cell density changes in the basal layer.

Related Experiment Videos

Analysis of mitotic spindle orientation in each cell layer during stratification.

Main Results:

Mitotic spindles were initially parallel to the basement membrane.
Spindle orientation shifted to predominantly vertical during layer formation.
Spindles returned to horizontal alignment after layer completion, a pattern repeated for successive layers.

Conclusions:

Dynamic changes in mitotic spindle orientation are integral to epithelial stratification.
These orientation shifts, alongside DNA synthesis and cell density, likely regulate layer formation.
The findings offer insights into corneal stratification and other stratified epithelia.