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Testis specific phosphoglycerate kinase B in mouse

J L VandeBerg, D W Cooper, P J Close

    The Journal of Experimental Zoology
    |November 1, 1976
    PubMed
    Summary
    This summary is machine-generated.

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    Mammals have two forms of phosphoglycerate kinase (PGK): X-linked PGK-A and autosomal PGK-B. PGK-B is crucial for sperm development and function, with distinct allelic variations observed in mice.

    Area of Science:

    • Biochemistry
    • Genetics
    • Mammalian Physiology

    Background:

    • Mammals express two genetically distinct phosphoglycerate kinase (PGK) isozymes: PGK-A and PGK-B.
    • PGK-A is typically detected in somatic and ovarian tissues, while PGK-B is prominent in male reproductive tissues.

    Purpose of the Study:

    • To investigate the expression patterns and allelic variations of PGK-B in mammals, specifically focusing on its role in male reproductive tissues.
    • To characterize the inheritance and biochemical properties of PGK-B allelic isozymes in mice.

    Main Methods:

    • Electrophoresis was used to detect and analyze PGK isozymes in various mammalian tissues.
    • Enzyme activity assays and heat stability tests were performed on different PGK-B variants.
    • Genetic crosses were utilized to study the inheritance patterns of PGK-B alleles in mice.

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    Main Results:

    • PGK-B activity is highly concentrated in testes, epididymides, and semen, appearing around day 22 of mouse development and increasing with germ cell proliferation.
    • Two autosomal codominant allelic isozymes of PGK-B in mice, designated F and S, were identified, differing in heat stability.
    • A potential third allelic isozyme of PGK-B with reduced activity and faster mobility was observed in some mouse strains.

    Conclusions:

    • PGK-B plays a significant role in male gamete development and function.
    • The allelic diversity of PGK-B in mice suggests complex genetic regulation and potential functional specialization.
    • Further research is warranted to elucidate the precise function and implications of the putative third PGK-B allelic isozyme.