The biotin requirement of rats fed 20% egg white

The biotin requirement of rats fed a diet based upon 62.4% sucrose, 20.0% egg white and 10.0% corn oil was determined using growth for 60 days as the cirterion. Maximal growth and maximal concentration of cholesterol in plasma occurred when the diet contained 2.00 mg biotin/kg. Growth depression was noted in one of two experiments in which 3.00 mg of biotin was present per kg of diet.