Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Helix bending as a factor in protein/DNA recognition

R E Dickerson1, T K Chiu

  • 1Oxford University Laboratory of Molecular Biophysics, UK. red@biop.ox.ac.uk

Biopolymers
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A standard reference frame for the description of nucleic acid base-pair geometry.

Journal of molecular biology·2001
Same author

Intrinsic bending and deformability at the T-A step of CCTTTAAAGG: a comparative analysis of T-A and A-T steps within A-tracts.

Journal of molecular biology·2001
Same author

DNA structure from A to B.

Proceedings of the National Academy of Sciences of the United States of America·2001
Same author

Mildly eccentric 'E-DNA'.

Nature structural biology·2001
Same author

1 A crystal structures of B-DNA reveal sequence-specific binding and groove-specific bending of DNA by magnesium and calcium.

Journal of molecular biology·2000
Same author

The structure of a stable intermediate in the A <--> B DNA helix transition.

Proceedings of the National Academy of Sciences of the United States of America·2000
Same journal

Untreated Rosehip Powder/Poly(Lactic Acid)/Poly(3-Hydroxybutyrate-Co-4-Hydroxybutyrate) Electrospun Mats for Wound Dressing Applications.

Biopolymers·2026
Same journal

Synthesis, Characterization, and Antidiabetic Evaluation of Sequence-Modified Liraglutide Analogs in a Drosophila melanogaster Model.

Biopolymers·2026
Same journal

Fabrication of an Antibacterial Alginate/Chitosan Hydrogel Dressing Loaded With CuO Nanoparticles for Wound Dressing Applications.

Biopolymers·2026
Same journal

Effect of Chitosan-Alginate Polyelectrolyte Complex Formation and Multilayer Polymer Configuration on the Characteristics of 3D-Printed Metronidazole-Loaded Periodontal Films.

Biopolymers·2026
Same journal

Phenolic Grafting of Oxidized Cellulose Nanofibers Using Ferulic Acid: Structural and Antioxidant Analysis Toward Bioactive Nanomaterials.

Biopolymers·2026
Same journal

Detection of a Target Nucleic Acid by Ligation-Assisted Fluorescence Enhancement of a Peptide Nucleic Acid (PNA) Twin Probe via Disulfide Binding.

Biopolymers·2026
See all related articles

B-DNA bending is primarily caused by roll angles between base pairs, not tilt. Three types of bending—localized kinking, smooth curvature, and 3D writhe—are identified, with roll compressing the major groove being most common.

Area of Science:

  • Structural Biology
  • Biophysics
  • Computational Biology

Background:

  • Normal vectors are crucial for analyzing B-DNA bending.
  • Previous studies lacked comprehensive analysis of DNA bending mechanisms.

Purpose of the Study:

  • To analyze DNA bending in protein/DNA complexes.
  • To identify distinct types and origins of B-DNA bending.

Main Methods:

  • Utilized normal vectors and angle matrices to study DNA structure.
  • Developed and applied the FREEHELIX program to 86 protein/DNA complexes.
  • Analyzed roll and tilt angles between adjacent base pairs.

Main Results:

  • B-DNA bending predominantly results from roll angles, with tilt having no net contribution.

Related Experiment Videos

  • Identified three bending types: localized kinking, smooth planar curvature, and 3D writhe.
  • Roll compressing the major groove is more frequent than minor groove compression.
  • Pyrimidine-purine steps are common in major kinking, while purine-purine steps show minimal roll.

    • Conclusions:

    • Roll angle is the primary determinant of B-DNA bending.
    • FREEHELIX program provides insights into DNA structural dynamics.
    • Understanding DNA bending is key to comprehending protein/DNA interactions.