Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

DNA recognition by structure-selective nucleases

D Suck1

  • 1Structural Biology Programme, European Molecular Biology Laboratory, Heidelberg, Germany.

Biopolymers
|January 1, 1997
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Structure of southern bean mosaic virus at 2.8 A resolution.

Nature·2009
Same author

RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex.

The EMBO journal·2001
Same author

Conformational flexibility in T4 endonuclease VII revealed by crystallography: implications for substrate binding and cleavage.

Journal of molecular biology·2001
Same author

The archaeal homolog of the Imp4 protein, a eukaryotic U3 snoRNP component.

Trends in biochemical sciences·2001
Same author

X-ray structure of T4 endonuclease VII: a DNA junction resolvase with a novel fold and unusual domain-swapped dimer architecture.

The EMBO journal·1999
Same author

Mutagenesis of conserved lysine residues in bacteriophage T5 5'-3' exonuclease suggests separate mechanisms of endo-and exonucleolytic cleavage.

Proceedings of the National Academy of Sciences of the United States of America·1999

This review explores DNA nucleases that recognize DNA structure, not sequence. Structural motifs in these enzymes explain their selective binding to DNA, including single-stranded, distorted, or complex DNA forms.

Area of Science:

  • Biochemistry
  • Structural Biology
  • Molecular Biology

Background:

  • Nucleases are enzymes that cleave DNA.
  • Sequence-specific nucleases are well-studied, but structure-specific nucleases also play crucial roles.
  • Understanding DNA-binding mechanisms is key to molecular biology.

Purpose of the Study:

  • To review nucleases that exhibit structural selectivity for DNA substrates.
  • To elucidate the structural basis for DNA recognition by these nucleases.
  • To highlight the diverse DNA structures recognized by various nucleases.

Main Methods:

  • Literature review of nucleases and their DNA substrates.
  • Analysis of structural data, including X-ray crystallography of nucleases and their DNA complexes.

Related Experiment Videos

  • Comparison of enzyme structures to understand substrate selectivity.

    • Main Results:

    • Nucleases P1 and S1 discriminate between single- and double-stranded DNA.
    • DNase I recognizes helical parameters like groove width and DNA flexibility.
    • Exonuclease III and HAP1 recognize abasic site distortions; 5'-nucleases and T4 endonuclease VII/RuvC recognize flap DNA and four-way junctions, respectively.
    • X-ray structures reveal specific structural motifs responsible for DNA recognition.

    Conclusions:

    • DNA nucleases employ structural recognition rather than sequence specificity.
    • Enzyme structural motifs are critical for selective binding to diverse DNA structures.
    • Structural insights explain the functional diversity of these important enzymes.