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Related Experiment Videos

17Beta-hydroxysteroid dehydrogenases in human bone cells

Y Dong1, Q Q Qiu, J Debear

  • 1Institute of Bone and Joint Disorders and Cancer, Bayer Corporation, West Haven, Connecticut 06516, USA.

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
|October 23, 1998
PubMed
Summary
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Bone cells can convert estrogens, with 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) primarily converting estradiol to less potent estrone. This suggests a role in regulating bone mass by modulating estrogen activity.

Area of Science:

  • Endocrinology and Bone Biology
  • Steroid Hormone Metabolism

Background:

  • Osteoblasts play a role in bone mass regulation.
  • Estrogen interconversion by bone cells is a potential mechanism.
  • 17beta-hydroxysteroid dehydrogenases (17beta-HSDs) are key enzymes in steroid metabolism.

Purpose of the Study:

  • To investigate the expression and activity of 17beta-HSDs in human osteoblasts (HOB) and osteosarcoma cell lines (MG63, TE85, SaOS-2).
  • To explore the role of these enzymes in estrogen interconversion within bone cells.

Main Methods:

  • Cell culture of human osteoblasts and osteosarcoma cell lines.
  • Measurement of 17beta-HSD activity in cell-free extracts.
  • Reverse transcription-polymerase chain reaction (RT-PCR) for mRNA expression analysis of 17beta-HSD isoforms.

Related Experiment Videos

  • Immunoblotting to detect protein expression of 17beta-HSD IV.

    • Main Results:

    • Significant 17beta-HSD activity was found in all bone cell types, with oxidation of estradiol to estrone being dominant.
    • 17beta-HSD II mRNA was detected in MG63, TE85, and HOB cells.
    • 17beta-HSD IV mRNA and protein were present in all analyzed bone cells, including both full-length and a truncated form.

    Conclusions:

    • Bone cells possess the enzymatic machinery to interconvert circulating estrogens.
    • The predominant activity suggests 17beta-HSDs primarily attenuate estradiol's action by converting it to the less potent estrone.
    • This interconversion may contribute to the regulation of bone mass.