Related Experiment Videos
cDNA excision in stable retroviral cDNA transfectants is prevented by R removal
R Rosin-Arbesfeld1, A Gazit, A Yaniv
1Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Biochemical and Biophysical Research Communications
|October 24, 1998
Abstract:
The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.