cDNA excision in stable retroviral cDNA transfectants is prevented by R removal
Related Experiment Video
Updated: Oct 23, 2026

Interview: HIV-1 Proviral DNA Excision Using an Evolved Recombinase
Published on: June 16, 2008
R Rosin-Arbesfeld1, A Gazit, A Yaniv
1Sackler School of Medicine, Tel Aviv University, Tel Aviv, 69978, Israel.
Abstract:
The present study provides evidence on the occurrence of DNA rearrangement between the redundant 5' and 3' R domains of equine infectious anemia virus (EIAV) Tat cDNA. This was correlated with a gradual loss of cDNA copy number concomitantly with a decrease in gene expression. Removal of the 5' RU5 abolished rearrangement and stabilized Tat expression in EIAV tat cDNA transfectants. Our data suggest that prior removal of the 5' R from cloned retroviral cDNAs can impede DNA rearrangement, thus preventing cDNA excision that frequently occurs and hinders permanent expression of retroviral cDNAs in stable transfectants.
Related Concept Videos
Retrovirus Life Cycles
Retroviruses
Conservative Site-specific Recombination and Phase Variation
The recognition sites for Cre recombinase called LoxP...
RACE - Rapid Amplification of cDNA Ends
Since the...

