Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Monocyte activation in platelet concentrates

D Grey1, W N Erber, K M Saunders

  • 1Transfusion Medicine Unit, Royal Perth Hospital, Australia.

Vox Sanguinis
|October 24, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The impacts of intensive mining on terrestrial and aquatic ecosystems: A case of sediment pollution and calcium decline in cool temperate Tasmania, Australia.

Environmental pollution (Barking, Essex : 1987)·2020
Same author

HER2 mRNA transcript quantitation in breast cancer.

Clinical & translational oncology : official publication of the Federation of Spanish Oncology Societies and of the National Cancer Institute of Mexico·2016
Same author

TGF-α and IL-6 plasma levels selectively identify CML patients who fail to achieve an early molecular response or progress in the first year of therapy.

Leukemia·2016
Same author

Water Security. Coping with the curse of freshwater variability.

Science (New York, N.Y.)·2014
Same author

Water security in one blue planet: twenty-first century policy challenges for science.

Philosophical transactions. Series A, Mathematical, physical, and engineering sciences·2013
Same author

Non-nodal type of mantle cell lymphoma is a specific biological and clinical subgroup of the disease.

Leukemia·2012
Same journal

Red cell alloantibodies in 71,525 Malaysian blood donations: Predominance of anti-Mia reflects GP.Mur-associated antigenic diversity.

Vox sanguinis·2026
Same journal

Barriers and enablers to non-remunerated plasma donation: A meta-synthesis of the qualitative literature using the theoretical domains framework.

Vox sanguinis·2026
Same journal

Haemolytic disease of the foetus and newborn due to anti-M: A systematic review.

Vox sanguinis·2026
Same journal

In vitro evaluation of apheresis platelet and plasma products collected and stored in non-DEHP disposable sets.

Vox sanguinis·2026
Same journal

Survey of national and regional rare donor programmes regarding Immunoglobulin A deficiency.

Vox sanguinis·2026
Same journal

Fibrinogen recovery in cryoprecipitate prepared from thawed plasma stored for 5 days post-thaw.

Vox sanguinis·2026
See all related articles

Monocytes in platelet concentrates show activation and are linked to increased IL-6. This suggests the manufacturing process may contribute to cytokine release, impacting transfusion reactions.

Area of Science:

  • Transfusion Medicine
  • Immunology
  • Hematology

Background:

  • Cytokines in platelet concentrates are implicated in febrile transfusion reactions.
  • Activated monocytes are a primary source of inflammatory cytokines.
  • The specific role of monocytes in cytokine production within platelet concentrates remains unclear.

Purpose of the Study:

  • To quantify monocytes in platelet concentrates.
  • To assess monocyte activation status.
  • To determine the association between monocyte presence and Interleukin-6 (IL-6) and Interleukin-1beta (IL-1beta) levels.

Main Methods:

  • Analysis of 17 platelet concentrates for total leukocyte and monocyte counts.
  • Measurement of monocyte-associated antigens CD14 and CD16.

Related Experiment Videos

  • Quantification of IL-1beta and IL-6 levels on storage days 1 through 5.

    • Main Results:

    • Monocytes in all analyzed platelet concentrates displayed increased CD14 and CD16 expression from day 1.
    • Elevated IL-6 levels were observed in 10 out of 17 platelet concentrates by day 3.
    • Higher initial monocyte counts correlated with increased IL-6 levels by day 5.

    Conclusions:

    • Monocytes within platelet concentrates exhibit signs of activation.
    • Monocyte activation appears to occur post-preparation, suggesting a role for the manufacturing process.
    • While higher monocyte counts are associated with increased IL-6 and IL-1beta, the degree of monocyte activation did not directly correlate with cytokine levels.