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Possible presynaptic inhibition in rat olfactory cortex

H G Pickles, M A Simmonds

The Journal of Physiology

|September 1, 1976

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Researchers identified a late negative wave in rat olfactory cortex slices, potentially mediated by GABAergic presynaptic inhibition. This finding offers insights into olfactory neurotransmission mechanisms.

Area of Science:

Neuroscience
Olfactory System Research
Electrophysiology

Background:

Field potentials in the rat olfactory cortex are crucial for understanding sensory processing.
Stimulation of the lateral olfactory tract (LOT) allows for in vitro investigation of neural circuit responses.

Purpose of the Study:

To investigate the nature and origin of a delayed negative wave (late N-wave) observed during slow stimulation of the rat olfactory cortex.
To elucidate the role of GABAergic neurotransmission in the generation of this late N-wave.

Main Methods:

Evoking field potentials in rat olfactory cortex slices using lateral olfactory tract stimulation at low frequencies (<0.1 Hz).
Utilizing modified artificial cerebrospinal fluid (ACSF) with low calcium/high magnesium to assess synaptic origins.

Related Experiment Videos

Administering the GABA antagonist bicuculline to evaluate GABAergic involvement.

Main Results:

A late N-wave, distinct from the primary N-wave, was observed, commencing 30 ms post-stimulus and lasting 50-150 ms.
Low Ca2+/high Mg2+ medium selectively abolished the late N-wave, suggesting a multisynaptic pathway.
Bicuculline eliminated the late N-wave without affecting the primary N-wave, implicating GABAergic mechanisms.
The late N-wave period showed reduced responses to a second stimulus, partly due to antidromic action potential collision.

Conclusions:

The late N-wave likely arises from a multisynaptic pathway involving GABAergic neurotransmission.
A presynaptic depolarizing action of a GABA-like transmitter may underlie presynaptic inhibition and the generation of the late N-wave.
These findings contribute to understanding inhibitory mechanisms within the olfactory cortex.