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[A modification to the chloroform-methanol method of isolating the matrix protein of the influenza virus]
A I Gordienlko1, Iu S Krivoshein, A I Gordienko
1Crimean Medical Institute, Department of Microbiology, Sympheropol', Ukraine.
Abstract:
A modification of chloroform-methanol method of isolation of M1 protein (M1P) of human influenza A virus allowed increasing 1.6 times the yield of the target product and decreasing 6.4 times the amount of organic solvents. It has been shown that M1 protein isolated by the modified method did not differ (as to its physicochemical characteristics and antigenic activity) from M1 protein isolated by means of the initial method.