Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Ectopic skeletal muscles derived from myoblasts implanted under the skin

A Irintchev1, J D Rosenblatt, M J Cullen

  • 1Department of Physiology, Neurophysiology, University of Bonn, Wilhelmstrasse 31, D-53111 Bonn, Germany.

Journal of Cell Science
|October 28, 1998
PubMed
Summary

Cultured myoblasts injected under the skin formed functional skeletal muscles in mice. These ectopic muscles, derived from implanted cells, show potential for myogenesis research and gene therapy.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Myxoedema coma.

Irish journal of medical science·2016
Same author

Response to 'Randomised controlled trials do not always give the results we want but that doesn't mean we should abandon randomised controlled trials'.

Spinal cord·2015
Same author

Motoneuron regeneration accuracy and recovery of gait after femoral nerve injuries in rats.

Neuroscience·2014
Same author

Selective correlations; not voodoo.

NeuroImage·2014
Same author

Revisiting multi-subject random effects in fMRI: advocating prevalence estimation.

NeuroImage·2013
Same author

Deficient functional recovery after facial nerve crush in rats is associated with restricted rearrangements of synaptic terminals in the facial nucleus.

Neuroscience·2013

Area of Science:

  • Regenerative Medicine
  • Muscle Biology
  • Tissue Engineering

Background:

  • Skeletal muscle regeneration is crucial for treating muscle-wasting diseases.
  • Understanding myogenesis in ectopic sites can reveal new therapeutic strategies.

Purpose of the Study:

  • To investigate the potential of cultured myoblasts to form functional skeletal muscle in an ectopic (non-native) site.
  • To characterize the morphology, phenotype, and function of these de novo generated muscles.

Main Methods:

  • Injection of cultured myoblasts (clonal or primary) into the subcutaneous lumbar region of adult mice.
  • Assessment of muscle formation, mass, and contractile properties over 7 weeks.
  • Histological, electron microscopic, and immunohistochemical analyses to evaluate muscle maturity and cell origin.

Related Experiment Videos

Main Results:

  • Distinct, functional skeletal muscles formed within 1-7 weeks post-injection, with clonal myoblasts yielding larger muscles.
  • Ectopic muscles exhibited spontaneous and evoked contractions, with properties similar to normal muscle.
  • Histological analysis confirmed mature muscle morphology, including aligned, capillarized fibers expressing muscle-specific proteins and innervated by host nerves.

Conclusions:

  • Cultured myoblasts can generate organized, functional skeletal muscle de novo in an ectopic subcutaneous site.
  • The host provides essential support tissues and innervation, facilitating muscle development.
  • Myogenic cells function as tissue-specific stem cells, capable of self-renewal and differentiation, supporting applications in myogenesis studies and gene delivery.