Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Structural studies of polymer-cushioned lipid bilayers

J Majewski1, J Y Wong, C K Park

  • 1Manuel Lujan, Jr. Neutron Scattering Center, Los Alamos National Laboratory, Los Alamos, New Mexico 87545, USA.

Biophysical Journal
|October 28, 1998
PubMed
Summary

Neutron reflectometry revealed that polymer cushioning affects lipid bilayer structure. Adsorbing vesicles first forms a bilayer, while pre-adsorbing polyethyleneimine (PEI) results in complex multilayer structures.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Prognostic Predictions for Patients with Glioblastoma after Standard Treatment: Application of Contrast Leakage Information from DSC-MRI within Nonenhancing FLAIR High-Signal-Intensity Lesions.

AJNR. American journal of neuroradiology·2019
Same author

A Compressed-Sensing Based Blind Deconvolution Method for Image Deblurring in Dental Cone-Beam Computed Tomography.

Journal of digital imaging·2018
Same author

Eliminating artifacts in single-grid phase-contrast x-ray imaging for improving image quality.

Computers in biology and medicine·2018
Same author

Effect of the sexual abstinence period recommended by the World Health Organization on clinical outcomes of fresh embryo transfer cycles with normal ovarian response after intracytoplasmic sperm injection.

Andrologia·2018
Same author

Type 2 diabetes mellitus and risk of open-angle glaucoma development in Koreans: An 11-year nationwide propensity-score-matched study.

Diabetes & metabolism·2017
Same author

Effect of rapamycin treatment during post-activation and/or in vitro culture on embryonic development after parthenogenesis and in vitro fertilization in pigs.

Reproduction in domestic animals = Zuchthygiene·2017

Area of Science:

  • Materials Science
  • Surface Chemistry
  • Biophysics

Background:

  • Lipid bilayers are fundamental to cell membranes.
  • Softly supported lipid bilayers offer model systems for studying membrane properties.
  • Controlling bilayer structure at interfaces is crucial for applications.

Purpose of the Study:

  • To investigate the structural impact of polymer cushioning on lipid bilayers at the quartz-solution interface.
  • To compare two preparation methods for polymer-cushioned lipid bilayers.
  • To elucidate the role of polyethyleneimine (PEI) in bilayer formation and support.

Main Methods:

  • Neutron reflectometry was employed to determine bilayer structure.
  • Lipid bilayers were formed from dimyristoylphosphatidylcholine (DMPC) vesicles.

Related Experiment Videos

  • Two distinct preparation protocols involving quartz substrates, PEI, and DMPC vesicles were utilized.

    • Main Results:

    • Adsorbing DMPC vesicles to bare quartz first, followed by PEI addition, resulted in an almost perfect bilayer lifted by the polymer.
    • Pre-adsorbing PEI to quartz before vesicle addition led to poor vesicle interaction and complex multilayer structures.

    Conclusions:

    • The order of deposition significantly influences the final structure of polymer-cushioned lipid bilayers.
    • Polyethyleneimine (PEI) can effectively cushion and lift lipid bilayers when introduced after vesicle adsorption.
    • Alternative deposition orders yield less ordered, potentially multilayered structures, highlighting the importance of preparation method.