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Human Bub1: a putative spindle checkpoint kinase closely linked to cell proliferation
1Department of Medicine, University of Cincinnati College of Medicine, Ohio 45267, USA.
Cell Growth & Differentiation : the Molecular Biology Journal of the American Association for Cancer Research
|October 28, 1998
Summary
Human Bub1 (hBub1) is a novel mitotic checkpoint kinase cloned from human cells. Its expression is restricted to proliferating cells, suggesting a role in cell cycle regulation and spindle checkpoint control.
Area of Science:
- Cell Biology
- Molecular Biology
- Genetics
Background:
- Eukaryotic cells possess a surveillance mechanism to delay anaphase until chromosomes are correctly aligned.
- Understanding this mitotic checkpoint is crucial for comprehending cell cycle regulation.
Purpose of the Study:
- To clone and characterize a human gene involved in the mitotic checkpoint.
- To investigate the role of the human Bub1 (hBub1) protein in cell cycle progression.
Main Methods:
- Full-length cDNA cloning of hBub1.
- Sequence comparison with yeast homologs (BUB1, MAD3).
- Northern blot, immunofluorescence, and antibody electroporation studies.
- Fluorescence in situ hybridization (FISH) for gene mapping.
Main Results:
- hBub1 shares homology with yeast BUB1 and MAD3, indicating conserved function.
- hBub1 mRNA is abundant in proliferating cells and downregulated during differentiation.
- hBub1 protein localizes to centromere-kinetochore regions and is essential for spindle checkpoint function.
- The hBub1 gene is mapped to chromosome 2q12-13.
Conclusions:
- hBub1 is a conserved mitotic checkpoint kinase crucial for regulating cell cycle progression.
- Its expression pattern suggests a role in proliferating cells.
- hBub1 cloning facilitates further research into spindle checkpoint control and potential roles in genetic disorders.