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BID, a proapoptotic BCL-2 family member, is localized to mouse chromosome 6 and human chromosome 22q11

K Wang1, X M Yin, N G Copeland

  • 1Departments of Medicine and Pathology, Washington University School of Medicine, Howard Hughes Medical Institute, St. Louis, Missouri, 63110, USA.

Genomics
|October 29, 1998
PubMed

Insights

The BCL-2 family member BID, a regulator of cell death, was isolated and characterized. Its gene (BID) was localized to human chromosome 22q11 and mouse Bid to chromosome 6.

Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • BID is a proapoptotic protein belonging to the BCL-2 family.
  • It contains a BH3 domain crucial for interactions with BCL-2 and BAX, mediating cell death.
  • Understanding BID's genetic basis and localization is key to its role in apoptosis.

Purpose of the Study:

  • To isolate and characterize the mouse Bid and human BID genes.
  • To determine the genomic structure of BID, including its exon count.
  • To map the chromosomal locations of Bid in mice and humans.

Main Methods:

  • Genomic DNA isolation and characterization.
  • Interspecific backcross analysis for gene mapping in mice.
  • Fluorescence in situ hybridization (FISH) for human gene localization.

Main Results:

  • Mouse Bid and human BID genomic clones were successfully isolated and characterized.
  • The human BID gene sequence is encoded within five exons.
  • Bid was mapped to the distal region of mouse chromosome 6 near the Atp6e locus.
  • Human BID was localized to chromosome 22q11, near the BCR gene.

Conclusions:

  • The genomic organization and chromosomal localization of BID have been elucidated.
  • This provides a foundation for further research into BID's function and its role in diseases.
  • The findings facilitate comparative mapping and understanding of apoptosis regulation across species.

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