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Second-site reversion analysis is not a reliable method to determine distances in membrane proteins: an assessment
1Department of Biology, University College London, Gower Street, London, WC1E 6BT, UK. b.meunier@ucl.ac.uk
Journal of Molecular Biology
|October 29, 1998
Abstract:
We have examined deficiency mutations and reversions in subunits I and II of yeast cytochrome c oxidase in order to test the reliability of second-site reversion analysis in prediction of tertiary structure of a membrane protein complex. It appears that the method can not provide information on distance between residues, since reversions can be up to 30 A from the primary mutations. However, the reversions are not randomly located in the structure but reveal regions essential for assembly or functional units.