Related Experiment Videos
High level expression and structural characterisation of herpes simplex virus type I transcriptional activator VP16
1Institut für Bakteriologie, Mykologie, und Hygiene, Veterinärmedizinische Universität Wien, Veterinaerplatz 1, Vienna, A-1210, Austria. Martin.Spatz@vu-wein.ac.at
Biochemical and Biophysical Research Communications
|October 29, 1998
Abstract:
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % alpha-helix and 17.5 % beta-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies.