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A technique for intravenous injection in the red-eared turtle (Pseudemys scripta elegans)

M G Vos-Maas, P Zwart

    Laboratory Animals
    |October 1, 1976
    PubMed
    Summary

    This study details a new technique for accessing the jugular vein in red-eared turtles. The method uses a specific anesthetic mixture, ensuring safe and effective surgical procedures for reptile research.

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    Area of Science:

    • Veterinary Medicine
    • Herpetology
    • Surgical Techniques

    Background:

    • The red-eared turtle (Trachemys scripta elegans) is a common model organism in biological research.
    • Accurate vascular access is crucial for experimental procedures in reptiles.
    • Existing methods for jugular vein access in turtles may be invasive or lack sufficient detail.

    Purpose of the Study:

    • To describe a reliable and effective technique for the exposure and injection of the jugular vein in red-eared turtles.
    • To establish optimal anesthetic parameters for surgical procedures involving the jugular vein in this species.
    • To provide a reproducible method for researchers working with red-eared turtles.

    Main Methods:

    • A surgical technique for jugular vein exposure and injection was developed.

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  • Anesthesia was achieved via intramuscular injection of a chloral hydrate and sodium pentobarbital mixture.
  • Dosage was standardized at 2.5 ml/kg bodyweight.

    • Main Results:

    • The anesthetic mixture provided satisfactory anesthesia and muscular relaxation for the procedure.
    • Induction time ranged from 30 to 45 minutes.
    • Surgical procedure duration was 30 to 45 minutes, with recovery times between 1 and 8 hours.

    Conclusions:

    • The described technique offers a safe and effective method for jugular vein access in red-eared turtles.
    • The anesthetic protocol ensures adequate patient preparation for vascular procedures.
    • This method facilitates further research requiring vascular access in this reptile model.