Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Affinity capillary electrophoresis: important application areas and some recent developments

N H Heegaard1, S Nilsson, N A Guzman

  • 1Department of Autoimmunology, Statens Serum Institut, Copenhagen, Denmark.

Journal of Chromatography. B, Biomedical Sciences and Applications
|October 29, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Low diagnostic and predictive value of anti-dsDNA antibodies in unselected patients with recent onset of rheumatic symptoms: results from a long-term follow-up Scandinavian multicentre study.

Scandinavian journal of rheumatology·2013
Same author

The use of selective adsorbents in capillary electrophoresis-mass spectrometry for analyte preconcentration and microreactions: a powerful three-dimensional tool for multiple chemical and biological applications.

Electrophoresis·2001
Same author

Association of beta2-glycoprotein I IgG and IgM antibodies with thrombosis and thrombocytopenia.

Lupus·2001
Same author

Conformational intermediate of the amyloidogenic protein beta 2-microglobulin at neutral pH.

The Journal of biological chemistry·2001
Same author

Distinct differences in autoantigen specificity of anti-neutrophil cytoplasm antibodies in systemic vasculitides and other inflammatory diseases.

The Israel Medical Association journal : IMAJ·2001
Same author

Monitoring the purity of a synthetic peptide by capillary electrophoresis: utilization of an on-line preconcentration method for improved separation and detection sensitivity.

Journal of capillary electrophoresis and microchip technology·2001
Same journal

High performance liquid chromatographic analysis of St. John's wort with photodiode array detection.

Journal of chromatography. B, Biomedical sciences and applications·2002
Same journal

Novel and simple two-step purification of a full-length rat glucocorticoid-receptor expressed in a baculovirus system.

Journal of chromatography. B, Biomedical sciences and applications·2002
Same journal

Stereoselective analysis of fluvastatin in human plasma for pharmacokinetic studies.

Journal of chromatography. B, Biomedical sciences and applications·2002
Same journal

Metabolism of methandrostenolone in the horse: a gas chromatographic-mass spectrometric investigation of phase I and phase II metabolism.

Journal of chromatography. B, Biomedical sciences and applications·2002
Same journal

Quantitative analysis of fentanyl in rat plasma by gas chromatography with nitrogen-phosphorus detection.

Journal of chromatography. B, Biomedical sciences and applications·2002
Same journal

Liquid chromatography-mass spectrometry assay of a thiadiazole derivative in mice: application to pharmacokinetic studies.

Journal of chromatography. B, Biomedical sciences and applications·2002
See all related articles

Affinity capillary electrophoresis (ACE) enables purification, quantitation, and binding constant estimation for diverse analytes. This technique, including molecular imprinting, is valuable for small molecules and biopolymers in biomedical research.

Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Separation Science

Background:

  • Affinity capillary electrophoresis (ACE) separates substances via specific or non-specific affinity interactions.
  • Interacting molecules can be free in solution or immobilized on a solid support.
  • ACE offers diverse applications with inherent advantages and disadvantages for each mode.

Purpose of the Study:

  • To review applications of ACE, focusing on purification, concentration, quantitation, and binding constant estimation.
  • To discuss molecular imprinting as a chemicoaffinity strategy in CE/CEC.
  • To explore ACE's utility for both small molecules and biopolymers, including proteins.

Main Methods:

  • Utilizes capillary electrophoresis with various affinity interactions (e.g., antigen-antibody, drug-protein).

Related Experiment Videos

  • Employs detectors such as UV, laser-induced fluorescence, and mass spectrometry.
  • Addresses background electrolyte selection and analyte quantitation strategies.

    • Main Results:

    • Demonstrates ACE's effectiveness for purifying and concentrating analytes from diluted or complex samples.
    • Shows ACE's capability for accurate analyte quantitation using calibration curves.
    • Highlights the estimation of binding constants through direct and derived binding curves.

    Conclusions:

    • ACE is a versatile technique applicable to a wide range of analytes, from small molecules to proteins.
    • Molecular imprinting represents a significant advancement in ACE/CEC chemicoaffinity strategies.
    • ACE holds substantial future potential in the biomedical field for characterization and analysis.