Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Videos

Engineering a lever into the kinesin neck

M Mazumdar¹, R A Cross

¹Molecular Motors Group, Marie Curie Research Institute, The Chart, Oxted, Surrey RH8 0TL, United Kingdom.

The Journal of Biological Chemistry

|October 29, 1998

Summary

Researchers investigated kinesin

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Non-opioid analgesic modes of pain management are associated with reduced postoperative complications and resource utilisation: a retrospective study of obstructive sleep apnoea patients undergoing elective joint arthroplasty.

British journal of anaesthesia·2018

Same author

Review: Mechanochemistry of the kinesin-1 ATPase.

Biopolymers·2016

Same author

Non-migratory tumorigenic intrinsic cancer stem cells ensure breast cancer metastasis by generation of CXCR4(+) migrating cancer stem cells.

Oncogene·2016

Same author

Sleep apnoea adversely affects the outcome in patients who undergo posterior lumbar fusion: a population-based study.

The bone & joint journal·2014

Same author

ROS-PIASγ cross talk channelizes ATM signaling from resistance to apoptosis during chemosensitization of resistant tumors.

Cell death & disease·2014

Same author

Perioperative database research: possibilities and pitfalls.

British journal of anaesthesia·2013

Area of Science:

Molecular Motor Function
Cellular Transport Mechanisms

Background:

Kinesin is a motor protein essential for intracellular transport.
The kinesin stepping mechanism and the potential role of a lever arm remain areas of active research.

Purpose of the Study:

To investigate the presence and function of a lever arm in the kinesin stepping mechanism.
To determine how structural modifications in the kinesin tail affect motor activity.

Main Methods:

Engineering a rodlike extension (alpha-actinin triple-coil repeats) into the kinesin tail at various positions.
Measuring the temperature dependence of microtubule gliding velocity using kinesin constructs.
Utilizing single-headed and dimeric kinesin constructs.

Main Results:

Inserting the rodlike module near the head-neck junction (Glu340) conferred motility to an immotile construct.
The module's effect on velocity was position-dependent, with greater impact closer to the head-neck junction.
The data suggest a short-throw lever arm may exist N-terminal to Val376, near the head-neck junction.

Conclusions:

The study provides evidence for a short-throw lever arm in kinesin, located close to the head-neck junction.
The position of structural elements in the kinesin tail significantly influences motor function and velocity.

Related Experiment Videos