Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Limitations of differential display

P Ledakis1, H Tanimura, T Fojo

  • 1Medicine Branch, National Cancer Institute, Bldg. 10, Rm. 12N226, 9000 Rockville Pike, Bethesda, Maryland, 20892, USA.

Biochemical and Biophysical Research Communications
|October 30, 1998
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Two cases of BRAF-mutated, bulbar conjunctival melanoma, and review of the published literature.

Clinical and experimental dermatology·2019
Same author

Small papular pseudolymphoma induced by a patch test for gold.

Clinical and experimental dermatology·2019
Same author

BRAF-mutated, acral verrucous melanoma successfully treated by dabrafenib plus trametinib combination therapy.

Clinical and experimental dermatology·2019
Same author

Diabetic scleroedema successfully treated by topical hyaluronidase injection: efficacy of magnetic resonance imaging.

Clinical and experimental dermatology·2018
Same author

Spontaneous endometriosis in cynomolgus monkeys as a clinically relevant experimental model.

Human reproduction (Oxford, England)·2018
Same author

Do patient access schemes for high-cost cancer drugs deliver value to society?-lessons from the NHS Cancer Drugs Fund.

Annals of oncology : official journal of the European Society for Medical Oncology·2017
Same journal

A transferrin receptor-based vector enables robust Type-II membrane protein display on mammalian cells.

Biochemical and biophysical research communications·2026
Same journal

NAT10-mediated RNA N4-acetylation promotes intestinal fibroblast senescence via DHRS2.

Biochemical and biophysical research communications·2026
Same journal

Deciphering potent MPL activation by the fucose-binding lectin thrombocorticin.

Biochemical and biophysical research communications·2026
Same journal

Mitochondrial genome alterations in cancer: From mutations and epigenetics to targeted therapiesack.

Biochemical and biophysical research communications·2026
Same journal

GPC3 chimeric antigen receptor (CAR)-NK cells combined with Enoblituzumab enhance the anti-tumor efficacy against hepatocellular carcinoma.

Biochemical and biophysical research communications·2026
Same journal

A miR-382-5p-PORCN axis modulates endogenous Wnt signaling during palatal development.

Biochemical and biophysical research communications·2026
See all related articles

Differential display PCR (DD-PCR) often fails to identify novel genes due to aberrant priming. This method can only detect abundantly expressed messages, limiting its utility for gene discovery.

Area of Science:

  • Molecular Biology
  • Genetics
  • Biotechnology

Background:

  • Differential display PCR (DD-PCR) is a widely used technique for identifying novel genes.
  • Despite its extensive application, DD-PCR has yielded limited success in discovering new genes of interest.

Purpose of the Study:

  • To investigate the reasons behind the frequent failures of differential display PCR.
  • To analyze the technical limitations that hinder the effectiveness of DD-PCR in gene identification.

Main Methods:

  • Experimental examination of DD-PCR protocols.
  • Analysis of PCR competition and primer binding.
  • Mathematical and computational assessment of detection limits.

Main Results:

Related Experiment Videos

  • Aberrant priming at both 5' and 3' ends of PCR products was identified as a major issue.
  • This aberrant priming leads to competition, preventing the detection of less abundant messages.
  • Calculations confirmed that only highly expressed genes are detectable with DD-PCR.

Conclusions:

  • DD-PCR is fundamentally limited in its ability to detect messages that are not abundantly expressed.
  • The technique's limitations, stemming from aberrant priming and competition, were predictable.
  • While DD-PCR may have niche applications, its broad utility for novel gene discovery is significantly restricted.