Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Bystander apoptosis triggers dendritic cell maturation and antigen-presenting function

P Rovere1, C Vallinoto, A Bondanza

  • 1Divisione di Medicina II, Istituto Scientifico H S. Raffaele and University of Milan, Italy. Rovere.Patrizia@hsr.it

Journal of Immunology (Baltimore, Md. : 1950)
|October 30, 1998
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Molecular constraints of sarcopenia in the ageing muscle.

Frontiers in aging·2025
Same author

Regulatory T cells expressing CD19-targeted chimeric antigen receptor restore homeostasis in Systemic Lupus Erythematosus.

Nature communications·2024
Same author

The immunology of the fetal-placental unit comes of age.

Clinical and experimental immunology·2019
Same author

Structural and functional brain connectomes in patients with systemic lupus erythematosus.

European journal of neurology·2019
Same author

CD44v6 as innovative sarcoma target for CAR-redirected CIK cells.

Oncoimmunology·2018
Same author

Jon van Rood (1926-2017).

Bone marrow transplantation·2017

Apoptotic cells, when not cleared efficiently, can trigger dendritic cell (DC) maturation. This process leads to the presentation of antigens, even without danger signals, highlighting a key immune surveillance mechanism.

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • Physiologic cell death via apoptosis normally occurs without inflammation or autoimmunity.
  • Dendritic cells (DCs) are professional antigen-presenting cells (APCs) crucial for initiating immune responses.

Purpose of the Study:

  • To investigate the interaction between apoptotic cells and dendritic cells (DCs).
  • To determine the consequences of apoptotic cell uptake by DCs on immune cell presentation and DC maturation.

Main Methods:

  • Exposure of DCs to varying numbers of apoptotic cells.
  • Analysis of antigen processing and presentation by DCs via MHC class I and class II pathways.
  • Assessment of DC maturation markers and cytokine secretion (IL-1beta, TNF-alpha).

Related Experiment Videos

Main Results:

  • DCs efficiently internalized and processed apoptotic cells for T cell presentation.
  • Antigen presentation efficiency correlated with the number of apoptotic cells.
  • High numbers of apoptotic cells induced DC maturation, including IL-1beta and TNF-alpha secretion.

Conclusions:

  • Failure in apoptotic cell clearance can lead to DC maturation.
  • DCs present intracellular antigens from apoptotic cells without external danger signals.
  • Apoptotic cell-induced DC maturation plays a role in immune surveillance and antigen presentation.