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Related Experiment Videos

Self-restricted dual receptor memory T cells

W T Lee1, V Shiledar-Baxi, G M Winslow

  • 1Wadsworth Center, Department of Biomedical Sciences, University at Albany School of Public Health, NY 12201, USA. william.lee@wadsworth.org

Journal of Immunology (Baltimore, Md. : 1950)
|October 30, 1998
PubMed
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Dual receptor CD4 T cells can develop memory responses to a new antigen after stimulation via a different receptor. This suggests unrelated antigens may maintain specific immune memory.

Area of Science:

  • Immunology
  • Cellular immunology
  • T cell memory

Background:

  • Enhanced immune responses during secondary exposure to antigens (Ag) are mediated by memory cells.
  • Dual receptor T cells possess two distinct T cell receptors (TCRs) for antigen recognition.

Purpose of the Study:

  • To investigate if stimulation of one receptor on dual receptor CD4 T cells can induce a memory response through the second receptor.
  • To explore the mechanism by which unrelated antigens might maintain specific immune memory.

Main Methods:

  • Generation of cloned T cells from dual receptor memory T cells.
  • Stimulation of cloned cells with specific antigens.
  • Analysis of cell proliferation and lymphokine secretion.
  • Characterization of antigen specificity and TCR usage.

Related Experiment Videos

  • Assessment of MHC restriction.

    • Main Results:

    • Stimulation via one receptor promoted memory responses through the second receptor, even without prior exposure to the second antigen.
    • Generated T cells proliferated and secreted lymphokines upon stimulation with either antigen.
    • Variants losing Ag specificity also lost the corresponding TCR, confirming independent recognition.
    • Antigen recognition was MHC class II restricted, recognizing self-MHC but not non-self-MHC molecules.

    Conclusions:

    • Dual receptor CD4 T cells can generate memory responses to previously unencountered antigens via a second TCR.
    • This cross-stimulation mechanism may represent a way to maintain specific immune memory through unrelated antigen encounters.