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Related Experiment Videos

cDNA sequence analysis, chromosomal assignment and expression pattern of the gene coding for integral membrane

K Pittois1, W Deleersnijder, J Merregaert

  • 1Department of Biochemistry, Laboratory of Molecular Biotechnology, University of Antwerp, Universiteitsplein 1, B-2610, Wilrijk, Belgium.

Gene
|October 31, 1998
PubMed
Summary

The integral membrane protein 2B (Itm2b) gene is widely expressed in mouse tissues, with three mRNA variants due to alternative polyadenylation. The human homolog (ITM2B) also codes for a 266-amino acid protein, similar to Itm2A.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • Integral membrane proteins play crucial roles in cellular functions.
  • The Itm2 family, including Itm2A, Itm2B, and Itm2C, are type II integral membrane proteins.
  • Previous studies identified Itm2A expression in osteo- and chondrogenic tissues.

Purpose of the Study:

  • To determine the complete cDNA sequence of the mouse integral membrane protein 2B gene (Itm2b).
  • To analyze the expression pattern and mRNA variants of Itm2b in mouse tissues.
  • To characterize the human homolog (ITM2B) and compare it with mouse Itm2b.

Main Methods:

  • Sequence analysis of expressed sequence tag (EST) clones and cDNA libraries.
  • 5' rapid amplification of cDNA ends (RACE) for full-length cDNA determination.

Related Experiment Videos

  • Northern blot analysis to assess tissue-specific gene expression.
  • Alignment of mouse and human EST data for sequence assembly.

    • Main Results:

    • The complete cDNA sequence of mouse Itm2b was determined, confirmed by EST alignment.
    • Itm2b is ubiquitously expressed in neonatal and adult mouse tissues.
    • Three distinct Itm2b mRNA variants result from alternative polyadenylation.
    • The human ITM2B gene codes for a 266-amino acid protein homologous to mouse Itm2b.
    • Both mouse Itm2b and human ITM2B genes map to conserved syntenic regions.

    Conclusions:

    • Mouse Itm2b exhibits ubiquitous expression with alternative polyadenylation, suggesting diverse regulatory mechanisms.
    • The high homology between mouse Itm2b and human ITM2B indicates conserved function across species.
    • Itm2b, as a member of the Itm2 family, likely plays a significant role in cellular processes, potentially beyond the osteo- and chondrogenic tissues where Itm2A is restricted.